Effect of intravitreal octreotide acetate injection on retinal neovascularization, morphology, and apoptotic cell death in an oxygen-induced retinopathy mouse model
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چکیده
Materials and methods: A total of 26 C57BL/6J mice were exposed to 75 ± 2% oxygen from postnatal day 7 to 12. On day 12, 12 mice (group C) were injected with 0.1 μg intravitreal octreotide acetate (IVOA) and 14 mice (group D) were injected with 0.05 μg IVOA, in the right eye. The contralateral eyes were injected with 1 μL isotonic saline (control group, group B). Four mice were used as negative controls (group A). Neovascularization was quantified by counting the number of retinal vascular endothelial cell nuclei anterior to the inner limiting membrane. Structural changes were examined by light and electron microscopy. Apoptosis was investigated using the TUNEL technique.
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